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Bibliografia publikacji pracowników
Państwowej Szkoły Wyższej w Białej Podlaskiej

Baza tworzona przez Bibliotekę Akademii Bialskiej im. Jana Pawła II.


Zapytanie: BUJALSKA-ZADROŻNY MAGDALENA
Liczba odnalezionych rekordów: 2



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Nr opisu: 0000036442
Autorzy: Piotr Poznański, Anna Leśniak, Magdalena Bujalska-Zadrożny, Joanna Strzemecka, Mariusz Sacharczuk.
Tytuł pracy: Bidirectional selection for high and low stress-induced analgesia affects G-protein activity
Tytuł czasopisma:
Szczegóły: 2019, Vol. 144, January 2019, p. 37--42
p-ISSN: 0028-3908
e-ISSN: 1873-7064
Charakterystyka formalna: artykuł w czasopiśmie zagranicznym
Charakterystyka merytoryczna: artykuł oryginalny naukowy
Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN)
Język publikacji: ENG
Wskaźnik Impact Factor ISI: 4.431
Punktacja ministerstwa: 140.000
Praca recenzowana
Słowa kluczowe ang.: antinociception ; opioid system ; HA and LA mice ; G-protein activation
Uwaga: Kopia dostępna w Sekcji Bibliometrii.
DOI: 10.1016/j.neuropharm.2018.10.014
Streszczenie: Mice selected for high (HA) and low (LA) swim stress-induced analgesia (SSIA) are a unique model for studying the genetic background of this phenomenon. HA and LA miceshow substantial differences in the magnitude of the antinociceptive response to stress and when treated with exogenous opioids. However, the direct cause underplaying this distinctive feature has not yet been identified. The current study was designed to investigate the possibility that disturbances in G-protein signaling could explain the divergent response to opioid agonists. Supraspinal and spinal opioid sensitivity was assessed in vivo with intraperitoneal morphine and subsequent thermal stimulus exposure. The level of opioid receptor-mediated G-protein activation was investigated by means of DAMGO and morphine-stimulated [35S]GTPγS assay in the brain and spinal cord homogenates from HA and LA mice. Morphine (3-249 ľmol/kg, i.p) was over 6 - and 3 - times more potent in HA than LA mice in the hot plate and tail-flick assays, respectively. Additionally, HA mice showed elevated ß - endorphin levels in the brain. Enhanced efficacy of agonist-stimulated [35S]GTPγS binding was detected in opioid receptor-rich limbic regions of HA mice like the hypothalamus and hippocampus. Increased G-protein activity also emerged in the thalamus, periaqueductal gray matter and prefrontal cortex. In conclusion, the magnitude of the antinociceptive response to opioids in HA and LA mice is correlated with alterations in G-protein activation in brain regions responsible for integration and descending modulation of nociceptive information as well as at sites governing the emotional response to stressful stimuli.

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Nr opisu: 0000036795
Autorzy: Anna Leśniak, Diana Chmielewska, Piotr Poznański, Magdalena Bujalska-Zadrożny, Joanna Strzemecka, Mariusz Sacharczuk.
Tytuł pracy: Divergent Response to Cannabinoid Receptor Stimulation in High and Low Stress-Induced Analgesia Mouse Lines Is Associated with Differential G-Protein Activation
Tytuł czasopisma:
Szczegóły: 2019, Vol. 404, p. 246--258
p-ISSN: 0306-4522
Charakterystyka formalna: artykuł w czasopiśmie zagranicznym
Charakterystyka merytoryczna: artykuł oryginalny naukowy
Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN)
Język publikacji: ENG
Wskaźnik Impact Factor ISI: 3.056
Punktacja ministerstwa: 140.000
Praca recenzowana
Słowa kluczowe ang.: cannabinoid system ; HA and LA mice ; G-protein activation ; WIN55,212-2
Uwaga: Kopia dostępna w Sekcji Bibliometrii.
https://www.sciencedirect.com/science/article/abs/pii/S0306452219301174
DOI: 10.1016/j.neuroscience.2019.02.015
Streszczenie: Bidirectional selection of mice for high (HA) and low (LA) swim stress-induced analgesia (SSIA) is associated with a divergent response to opioids. In the current study, we investigated whether the genetic divergence in opioid system activity between HA and LA mice also affects cannabinoid sensitivity. Additionally, we also investigated whether the endocannabinoid system mediates SSIA in these lines. Numerous reports support the existence of pharmacological and molecular interactions between the opioid and cannabinoid systems along the pain pathways, as both systems utilize the same G-protein subtype for signal transduction. Mice from both lines were treated with a non-selective CB1/CB2 agonist, WIN55,212-2 and their behavior was evaluated according to the tetrad paradigm assessing antinociception, catalepsy, hypothermia and locomotor activity. Surprisingly, the engagement of CB1 receptors in SSIA was not confirmed. G-protein activation was studied in different brain regions and the spinal cord in the [35S]GTP?S assay. It was shown that WIN55,212-2 produced more potent antinociception in HA than in LA mice. Also, HA mice displayed stronger cannabinoid-induced catalepsy in the bar test. However, LA mice were more sensitive to the hypothermic effect of WIN55,212-2. The intensity of behavioral responses to WIN55,212-2 was correlated with increased G-protein activation in the periaqueductal gray matter, frontal cortex, striatum and thalamus in HA mice. A weak response to WIN55,212-2 in LA mice could depend on impaired CB2 receptor signaling. In conclusion, differences in both opioid and cannabinoid sensitivity between HA and LA mice could stem from alterations in intracellular second messenger mechanisms involving G-protein activation.

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