Bibliografia publikacji pracowników
Państwowej Szkoły Wyższej w Białej Podlaskiej
Baza tworzona przez Bibliotekę Akademii Bialskiej im. Jana Pawła II.
Zapytanie:
RADULSKI ŁUKASZ Liczba odnalezionych rekordów: 7
Przejście do opcji zmiany formatu | Wyświetl/ukryj etykiety | Wyświetlenie wyników w wersji do druku | Pobranie pliku do edytora | Nowe wyszukiwanie Streszczenie: Over the last 30 years, the number of invasive turtle species living in the wild has significantly increased in Poland. This proliferation carries many threats, which mainly include the displacement of native species of animals from their natural habitats. Turtles can also be reservoirs for pathogens, including bacteria from the Mycobacterium genus. In order to confirm or rule out the presence of acid-fast mycobacteria in the population of invasive turtle species, samples from carapace, plastron, internal organs and mouth cavity swabs from 125 animals were tested. Twenty-eight mycobacterial strains were isolated in culture, which were classified as atypical following multiplex-PCR reactions. The GenoType Mycobacterium Common Mycobacteria (CM) test, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, PCR-restriction fragment length polymorphism (PRA)-hsp65 and DNA sequencing were used to identify the species of isolates. Of the 28 strains, 11 were identified as M. fortuitum, 10 as M. chelonae, 3 as M. avium ssp. avium, 2 as M. nonchromogenicum and 1 each of M. neoaurum and M. scrofulaceum. The results of the research will also strengthen the understanding that these animals can be vectors for pathogens when living in the wild.
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Nr opisu: 0000045178 Autorzy: Monika Krajewska-Wędzina, Michał K. Krzysiak, Małgorzata Bruczyńska, Blanka Orłowska, Anna Didkowska, Łukasz Radulski, Jan Wiśniewski, Wanda Olech, Aneta Nowakiewicz, Mirosław Welz, Stanisław Kaczor, Marcin Weiner, Krzysztof Anusz. Tytuł pracy: Ten Years of Animal Tuberculosis Monitoring in Free-Living European Bison (Bison bonasus) in Poland Tytuł czasopisma: Szczegóły: 2023, Vol. 13, issue 7, article number 1205 p-ISSN: 2076-2615 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 3.000 Punktacja ministerstwa: 100.000 Słowa kluczowe ang.: animal tuberculosis ; European bison ; MTBC ; Mycobacterium bovis ; Mycobacterium avium spp. homonissuis ; Poland https://www.mdpi.com/2076-2615/13/7/1205 DOI: 10.3390/ani13071205 Streszczenie: In the period 1996-2012, two outbreaks of animal tuberculosis were noted in the population of free-living European bison (Bison bonasus caucasicus) in the Bieszczady Mountains, Southern Poland. As the European bison is an endangered species and particularly susceptible to tuberculosis, not to mention a national icon, the decision was made to test all deceased bison for TB in Poland. The screened bison were obtained by elimination due to poor health or natural death. A total of 159 European bison have been examined over the last 10 years. The individuals came from four regions of Poland (Białowieża Forest, Bieszczady Mountains, Borecka Forest, Knyszyńska Forest), not only from the area where tuberculosis is still endemic. Mycobacterium bovis and Mycobacterium avium spp. hominisuis were identified in two different herds. The isolation of M. bovis from European bison was the first case described in Poland. So far, the only causative agent of tuberculosis identified in European bison in Poland, both in the wild and in captive herds, was Mycobacterium caprae. The isolated M. bovis spoligotype has not previously been registered in international spoligotype databases so far. The obtained results highlight the need to monitor TB in European bison in Poland.
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Nr opisu: 0000045120 Autorzy: Mirosław Welz, Monika Krajewska-Wędzina, Blanka Orłowska, Anna Didkowska, Łukasz Radulski, Przemysław Łoś, Marcin Weiner, Krzysztof Anusz. Tytuł pracy: The eradication of M. caprae tuberculosos in wild boar (Sus scrofa) in the Bieszczady Mountains, southern Poland - an administrative perspective Tytuł czasopisma: Szczegóły: 2023, Vol. 67, issue 1, p. 61--66 p-ISSN: 2450-7393 e-ISSN: 2450-8608 Charakterystyka formalna: artykuł w czasopiśmie polskim Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 1.800 Punktacja ministerstwa: 200.000 Słowa kluczowe ang.: Mycobacterium caprae ; spoligotype SB2391 ; tuberculosis ; veterinary administration ; wild boar https://sciendo.com/article/10.2478/jvetres-2023-0006 DOI: 10.2478/jvetres-2023-0006 Streszczenie: Animal tuberculosis (TB) is a zoonotic desease caused by acid-fast bacteria belonging to the Mycobacterium tuberculosis complex (MTBC). Both animals and humans are susceptible to infection by the MTBC. Interspecies transmission is also possible, including to livestock and humans. In the years 1997-2013, many tuberculosis cases were recorded in European bison in the Bieszczady Mountains; more alarmingly, TB was also recorded in wild boar in the years 2013-2020. Matherial and Methods: In the years 2013-2020, 104 wild boar from the Bieszczady Mountains were tested for TB through necropsy, mycobacterial culture, strain identification and spoligotyping. Results: The microbiological examination confirmed TB in 46 wild boar; these infectios were identified as M. caprae, spoligotype SB2391. Conclusion: Free-living European bison are at risk of TB infection from wild boar carrying M. caprae. This situation also poses a risk to local cattle. There is a need for further activities aimed at monitoring the disease, preventing further transmission, and minimising the risk to public health.
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Nr opisu: 0000044251 Autorzy: Monika Krajewska-Wędzina, Łukasz Radulski, W. Ray Waters, Anna Didkowska, Anna Zabost, Ewa Augustynowicz-Kopeć, Sylwia Brzezińska, Marcin Weiner. Tytuł pracy: Mycobacterium bovis Transmission between Cattle and a Farmer in Central Poland Tytuł czasopisma: Szczegóły: 2022, Vol. 11, issue 10, article number 1170 p-ISSN: 2076-0817 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 3.700 Punktacja ministerstwa: 100.000 Słowa kluczowe ang.: cattle ; human ; Mycobacterium bovis ; SB0120 ; transmission ; Poland https://www.mdpi.com/2076-0817/11/10/1170 DOI: 10.3390/pathogens11101170 Streszczenie: Zoonoses have recently become an increasing public health problem. Zoonoses are estimated to account for 60% of all emerging infectious diseases. One particularly important zoonosis is human tuberculosis, especially tuberculosis due to Mycobacterium bovis (M. bovis), which is naturally resistant to pyrazinamide (PZA). Material and Methods: The patient had a pulmonary form of tuberculosis accompanied by a cough and fever. At the same time, the disease was also confirmed in 20 out of 25 cattle on the farm. The clinical specimen (sputum) was examined in accordance with the European Union (EU) laboratories' methodology. Tissue materials from cattle were verified in the National Veterinary Research Institute (NVRI), in the Bovine tuberculosis (BTB) Reference Laboratory, Pulawy, Poland and tested in accordance with the guidelines for the laboratory diagnosis of BTB. Results: All M. bovis isolates represented one spoligotype, SB0120. The results of mycobacterial interspersed repetitive unit variable number tandem repeat (MIRU-VNTR) evaluation showed the same genetic pattern. Conclusion: Findings from this study suggest the first confirmed interspecific transmission of Mycobacterium bovis, between a farmer and his cattle, in Poland. Present findings support the increasing concern regarding zoonotic TB that has been highlighted elsewhere.
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Nr opisu: 0000043434 Autorzy: Monika Krajewska-Wędzina, Michele A. Miller, Anna Didkowska, Anna Kycko, Łukasz Radulski, Marek Lipiec, Marcin Weiner. Tytuł pracy: The potential risk of international spread of Mycobacterium bovis associated with movement of alpacas Tytuł czasopisma: Szczegóły: 2022, Vol. 66, issue 1, p. 53--59 p-ISSN: 2450-7393 e-ISSN: 2450-8608 Charakterystyka formalna: artykuł w czasopiśmie polskim Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 1.800 Punktacja ministerstwa: 140.000 Słowa kluczowe ang.: alpaca ; Enferplex Camelid TB test ; Mycobacterium bovis ; tuberculin skin test https://sciendo.com/article/10.2478/jvetres-2022-0012 DOI: 10.2478/jvetres-2022-0012 Streszczenie: The study highlights the transboundary nature of tuberculosis (TB) in alpacas and the failure of current ante-mortem testing protocols (the tuberculin skin and Enferplex Camelid TB tests) to identify TB-free alpaca herds and individuals for export. Our research and the available literature indicate that the alpaca (Vicugna pacos) is extremely susceptible to Mycobacterium bovis infection, and that testing periodicity fails to take into account that animals do not manifest disease symptoms for a long time. The skin test failed to identify Mycobacterium bovis infection in two alpacas prior to their movement from the UK to Poland. The animals were purchased by a breeding centre in Poland, and were then shown at an international animal exhibition. The last owner of the alpacas before their deaths from TB bought the infected animals unwittingly in order to run rehabilitation activities with disabled children on his farm. Material and Methods: Thoracic lymph node, lung and liver tissue samples obtained at necropsy were examined histopathologically after Ziehl-Neelsen staining. Tissue samples were homogenised and mycobacteria present there were cultured on Stonebrink's medium during a 6-week incubation. A commercial test using polymorphism of the chromosomal direct repeat region provided species identification and additional identification was by spacer oligonucleotide typing and mycobacteria interspersed repetitive unit-variable number tandem repeat analysis with a gel electrophoresis protocol. Results: The microbiological examination confirmed multiorgan TB caused by the SB0666 spoligotype of Mycobacterium bovis. Conclusion: Due to the suboptimal performance of current diagnostic tests for TB in alpacas, there is a risk that infected animals may be moved unwittingly. A risk of TB spread associated with the international movement of alpacas is implied by this study.
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Nr opisu: 0000042719 Autorzy: Monika Krajewska-Wędzina, Marcin Weiner, Marek Lipiec, Łukasz Radulski, Krzysztof Szulowski. Tytuł pracy: Epizootic assessment of mycobacterium avium spp. Paratuberculosis infections in cattle in Poland in 2011-2020 as a potential etiological factor of Crohn's disease in humans Tytuł równoległy: Ocena epizootyczna zakażeń mycobacterium avium spp. Paratuberculosis u bydła w Polsce w latach 2011-2020 jako potencjalny czynnik etiologiczny choroby Leśniowskiego-Crohna u ludzi Tytuł czasopisma: Szczegóły: 2021, Vol. 15, issue 4, p. 323--327 p-ISSN: 2353-6942 e-ISSN: 2354-0265 Charakterystyka formalna: artykuł w czasopiśmie polskim Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie bez IF (wykaz MEiN) Język publikacji: ENG Punktacja ministerstwa: 20.000 Słowa kluczowe: bydło ; choroba Leśniowskiego-Crohna ; Mycobacterium avium spp. paratuberculosis ; paratuberkuloza ; zdrowie publiczne Słowa kluczowe ang.: cattle ; Crohn's disease ; Mycobacterium avium spp. paratuberculosis ; paratuberculosis ; public health https://www.termedia.pl/Epizootic-assessment-of-Mycobacterium-avium-spp-paratuberculosis-infections-in-cattle-in-Poland-in-2011-2020-as-a-potential-etiological-factor-of-Crohn-s-disease-in-humans,99,4 DOI: 10.5114/hpc.2021.110458 Streszczenie: Introduction: Mycobacterium avium spp. paratuberculosis (MAP) is a bacterial germ and the etiologic agent in paratuberculosis also known as Johne's disease, a debilitating disease seen in animals. MAP is one of the triggers in the development of Crohn's disease in humans. Therefore the presence of these microorganisms in the environment and their prevalence in farm animals in relation to Crohn's disease is a new area of research. To date, it has not been evaluated in Poland. Material and methods: The materials for this study consisted of 14,468 samples of bovine serum across Poland. Determination of anti-MAP antibody levels was performed using an immunoenzymatic Paratuberculosis Screening Ab Test p/n P07130-5Ž (IDEXX, Westbrook, Maine 04092, United States). Results: The study consisted of 14,468 samples of bovine serum. Anti-MAP antibodies were identified in 349 cattle sera. Conclusions: Successful paratuberculosis screening programs can lead to a reduction of MAP in animal products. As the role of MAP in Crohn's disease is not fully understood, future research should be conducted to address this knowledge gap and avoid a potential zoonotic public health problem. Streszczenie: Wstęp: Mycobacterium avium spp. paratuberculosis (MAP) zwany także prątkiem Johnego jest czynnikiem etiologicznym paratuberkulozy, wyniszczającej choroby występującej u zwierząt. Jednym z czynników niezbędnych do rozwoju choroby Crohna u ludzi jest obecność MAP, stąd też obecność w środowisku tych drobnoustrojów, a przede wszystkim ocena ich występowania u zwierząt gospodarskich w odniesieniu do choroby Crohna, stanowi nowy obszar badań, nieopisany dotąd w Polsce. Materiał i metody: Materiał do badań stanowiło 14468 bydlęcych surowic pochodzących z terenu całej Polski. Oznaczenie poziomu przeciwciał anty-MAP wykonano przy użyciu immunoenzymatycznego testu Paratuberculosis Screening Ab Test p/n P07130-5Ž (IDEXX, Westbrook, Maine 04092, United States). Wyniki: W badanej grupie 14468 surowic, przeciwciała anty-MAP potwierdzono u 349 sztuk bydła. Wnioski: Udane programy kontroli paratuberkulozy mogą prowadzić do zmniejszenia występowania MAP w produktach zwierzęcego pochodzenia. W związku z tym, że rola MAP nie jest do końca poznana w chorobie Crohna, przyszłe badania powinny zająć się tymi lukami, aby uniknąć potencjalnego odzwierzęcego problemu zdrowia publicznego.
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Nr opisu: 0000039361 Autorzy: Monika Krajewska-Wędzina, Monika Kozińska, Łukasz Radulski, Marek Lipiec, Ewa Augustynowicz-Kopeć, Marcin Weiner, Krzysztof Szulowski. Tytuł pracy: Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland Tytuł czasopisma: Szczegóły: 2020, Vol. 64, issue 1, p. 45--50 p-ISSN: 2450-7393 e-ISSN: 2450-8608 Charakterystyka formalna: artykuł w czasopiśmie polskim Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 1.744 Punktacja ministerstwa: 140.000 Praca recenzowana Słowa kluczowe ang.: cattle ; bovine tuberculosis ; Mycobacterium bovis ; Poland Uwaga: Kopia dostępna w Sekcji Bibliometrii. Inne bazy podające opis:
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https://content.sciendo.com/view/journals/jvetres/64/1/article-p45.xml DOI: 10.2478/jvetres-2020-0003 Streszczenie: Introduction. Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008-2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks. Material and Methods. The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province. Results. Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.
