Bibliografia publikacji pracowników
Państwowej Szkoły Wyższej w Białej Podlaskiej
Baza tworzona przez Bibliotekę Akademii Bialskiej im. Jana Pawła II.
Zapytanie:
SACHARCZUK MARIUSZ Liczba odnalezionych rekordów: 12
Przejście do opcji zmiany formatu | Wyświetl/ukryj etykiety | Wyświetlenie wyników w wersji do druku | Pobranie pliku do edytora | Nowe wyszukiwanie Streszczenie: In a healthy physiological state, the mucous membrane of the oral cavity creates a suitable environment for the colonization of Candida spp. yeasts. The aim of the study was to analyze the nanomechanical properties of C. albicans cells derived from the oral cavity of healthy people in a biofilm produced in laboratory conditions. Candida spp. were sampled from the oral cavity of healthy individuals. The process of biofilm formation was analyzed using classic microscopic observation enriched with SEM (scanning electron microscope) and the nanomechanical properties of the cells were assessed with the use of the atomic force microscopy technique (AFM). From all isolated strains in the samples collected of the oral cavity healthy people was detected 79% C. albicans. Other isolated species belonged to the group "non-albicans". The observations of C. albicans carried out in 24-h cultures revealed a tendency of the cells to form a biofilm structure with multilayer cell systems. The diameter of C. albicans cells in this structure was 5.75 ľm, and the length of the pseudohyphae was 17.08 ľm. The presence of an extracellular substance surrounding the C. albicans cells was detected. The mean value of the adhesion force determined for C. albicans cells was 4.01 nN. Areas with increased hardness (Force Modulation Mode signal; FMM signal) were found mainly in the zones of cells in contact with the glass substrate. The analysis of Candida cells in liquid samples gives satisfactory results, as it prevents unfavorable changes in the cell surface and thus provides more reliable results. The quality of the biofilm is probably related to the nanomechanical properties of C. albicans cells and may consequently contribute to the stability of the biofilm structures and their susceptibility or resistance to antifungal drugs. The presence of Candida spp. especially in companion animals (dogs, cats) poses a risk of their transmission to the human organism. For this reason, it is advisable to undertake additional research to analyze the ability of zoonotic-origin Candida spp. to form biofilms with comparison of the biofilm-formation capacity of species isolated from humans. Projekt/grant: Zastosowanie mikroskopu sił atomowych (AFM) w ocenie własności nanomechanicznych Candida spp : Fundusz Grantów na Badania Własne / PSW Biała Podlaska, PB/1/2019
2/12
Nr opisu: 0000046722 Autorzy: Patrycja Teodorowicz, Małgorzata Tokarska-Rodak, Estera Michaluk, Marta Zarębska, Dorota Plewik, Tomasz Marek Grudniewski, Mariusz Sacharczuk. Tytuł pracy: Assessment of nanomechanical properties of Candida albicans as an element of the oral mycobiota in healthy subjects Tytuł całości: W: Slovenian Microbiome Network Symposium 2023 "Defining a Healthy Microbiome", 30.11-1.12.2023, Maribor, Slovenia : abstract book / ed. Nejc Stopniek, Sandra Janeič Miejsce wydania: Maribor Wydawca: National Laboratory of Health, Environment and Food Rok wydania: 2023 Strony zajęte przez pracę: P. 66-67 Charakterystyka formalna: streszczenie zjazdowe międzynarodowe (książka streszczeń) Charakterystyka merytoryczna: konferencja naukowa międzynarodowa Język publikacji: ENG Słowa kluczowe ang.: Candida albicans ; healthy people ; nanomechanical properties ; oral mycobiota https://slovenianmicrobiome.net/abstract_book/
3/12
Nr opisu: 0000046416 Autorzy: Norwin Kubick, Justyna Paszkiewicz, Irmina Bieńkowska, Michał Ławiński, Jarosław Olav Horbańczuk, Mariusz Sacharczuk, Michel Edward Mickael. Tytuł pracy: Investigation of Mutated in Colorectal Cancer (MCC) Gene Family Evolution History Indicates a Putative Role in Th17/Treg Differentiation Tytuł czasopisma: Szczegóły: 2023, Vol. 24, issue 15, article number 11940 p-ISSN: 1422-0067 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 5.600 Punktacja ministerstwa: 140.000 Słowa kluczowe ang.: colorectal cancer ; differentiation ; evolution ; Th17 ; Treg https://www.mdpi.com/1422-0067/24/15/11940 DOI: 10.3390/ijms241511940 Streszczenie: The MCC family of genes plays a role in colorectal cancer development through various immunological pathways, including the Th17/Treg axis. We have previously shown that MCC1 but not MCC2 plays a role in Treg differentiation. Our understanding of the genetic divergence patterns and evolutionary history of the MCC family in relation to its function, in general, and the Th17/Treg axis, in particular, remains incomplete. In this investigation, we explored 12 species' genomes to study the phylogenetic origin, structure, and functional specificity of this family. In vertebrates, both MCC1 and MCC2 homologs have been discovered, while invertebrates have a single MCC homolog. We found MCC homologs as early as Cnidarians and Trichoplax, suggesting that the MCC family first appeared 741 million years ago (Ma), whereas MCC divergence into the MCC1 and MCC2 families occurred at 540 Ma. In general, we did not detect significant positive selection regulating MCC evolution. Our investigation, based on MCC1 structural similarity, suggests that they may play a role in the evolutionary changes in Tregs' emergence towards complexity, including the ability to utilize calcium for differentiation through the use of the EFH calcium-binding domain. We also found that the motif NPSTGE was highly conserved in MCC1, but not in MCC2. The NPSTGE motif binds KEAP1 with high affinity, suggesting an Nrf2-mediated function for MCC1. In the case of MCC2, we found that the "modifier of rudimentary" motif is highly conserved. This motif contributes to the regulation of alternative splicing. Overall, our study sheds light on how the evolution of the MCC family is connected to its function in regulating the Th17/Treg axis. Projekt/grant: Wpływ endogennego rozszczelnienia bariery krew-mózg w patogenezie zróżnicowanej podatności na inokulowanego czerniaka u linii myszy selekcjonowanych w kierunku wysokiej i niskiej analgezji postresowej : Akademia Bialska im. Jana Pawła II w ramach Regulaminu wsparcia rozwoju zawodowego pracowników, PB/5/2022
4/12
Nr opisu: 0000044765 Autorzy: Suniti Bhumik, Marzena Łazarczyk, Norwin Kubick, Pavel Klimovich, Agata Gurba, Justyna Paszkiewicz, Patrycja Teodorowicz, Tomasz Kocki, Jarosław Olav Horbańczuk, Gina Manda, Mariusz Sacharczuk, Michel-Edwar Mickael. Tytuł pracy: Investigation of the Molecular Evolution of Treg Suppresion Mechanisms Indicates a Covergent Origin Tytuł czasopisma: Szczegóły: 2023, Vol. 45, issue 1, p. 628--648 p-ISSN: 1467-3045 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 3.100 Punktacja ministerstwa: 70.000 Słowa kluczowe ang.: ai ; evolution ; molecular evolution ; tregs https://www.mdpi.com/1467-3045/45/1/42 DOI: 10.3390/cimb45010042 Streszczenie: Regulatory T cell (Treg) suppression of conventional T cells is a central mechanism that ensures immune system homeostasis. The exact time point of Treg emergence is still disputed. Furthermore, the time of Treg-mediated suppression mechanisms' emergence has not been identified. It is not yet known whether Treg suppression mechanisms diverged from a single pathway or converged from several sources. We investigated the evolutionary history of Treg suppression pathways using various phylogenetic analysis tools. To ensure the conservation of function for investigated proteins, we augmented our study using nonhomology-based methods to predict protein functions among various investigated species and mined the literature for experimental evidence of functional convergence. Our results indicate that a minority of Treg suppressor mechanisms could be homologs of ancient conserved pathways. For example, CD73, an enzymatic pathway known to play an essential role in invertebrates, is highly conserved between invertebrates and vertebrates, with no evidence of positive selection (w = 0.48, p-value < 0.00001). Our findings indicate that Tregs utilize homologs of proteins that diverged in early vertebrates. However, our findings do not exclude the possibility of a more evolutionary pattern following the duplication degeneration-complementation (DDC) model. Ancestral sequence reconstruction showed that Treg suppression mechanism proteins do not belong to one family; rather, their emergence seems to follow a convergent evolutionary pattern.
5/12
Nr opisu: 0000045084 Autorzy: Marzena Łazarczyk, Michel Edward Mickael, Dominik Skiba, Ewa Kurzejamska, Michał Ławiński, Jarosław Olav Horbańczuk, Jakub Radziszewski, Karolina Fraczek, Renata Wolińska, Justyna Paszkiewicz, Piotr Religa, Mariusz Sacharczuk. Tytuł pracy: The Journey of Cancer Cells to the Brain : Challenges and Opportunities Tytuł czasopisma: Szczegóły: 2023, Vol. 24, issue 4, article number 3854 p-ISSN: 1422-0067 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 5.600 Punktacja ministerstwa: 140.000 Słowa kluczowe ang.: braine ; cancer ; immune cells ; metastasis https://www.mdpi.com/1422-0067/24/4/3854 DOI: 10.3390/ijms24043854 Streszczenie: Cancer metastases into the brain constitute one of the most severe, but not uncommon, manifestations of cancer progression. Several factors control how cancer cells interact with the brain to establish metastasis. These factors include mediators of signaling pathways participating in migration, infiltration of the blood-brain barrier, interaction with host cells (e.g., neurons, astrocytes), and the immune system. Development of novel therapies offers a glimpse of hope for increasing the diminutive life expectancy currently forecasted for patients suffering from brain metastasis. However, applying these treatment strategies has not been sufficiently effective. Therefore, there is a need for a better understanding of the metastasis process to uncover novel therapeutic targets. In this review, we follow the journey of various cancer cells from their primary location through the diverse processes that they undergo to colonize the brain. These processes include EMT, intravasation, extravasation, and infiltration of the blood-brain barrier, ending up with colonization and angiogenesis. In each phase, we focus on the pathways engaging molecules that potentially could be drug target candidates.
6/12
Nr opisu: 0000044217 Autorzy: Marzena Lazarczyk, Kamil Duda, Michel Edward Mickael, Onurhan AK, Justyna Paszkiewicz, Agnieszka Kowalczyk, Jarosław Olav Horbańczuk, Mariusz Sacharczuk. Tytuł pracy: Adera 2.0. A Drug Repurposing Workflow for Neuroimmunological Investigations Using Neural Networks Tytuł czasopisma: Szczegóły: 2022, Vol. 27, issue 19, article number 6453 p-ISSN: 1420-3049 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 4.600 Punktacja ministerstwa: 140.000 Słowa kluczowe ang.: deep neural network ; drug repurposing ; neuro-immunology https://www.mdpi.com/1420-3049/27/19/6453 DOI: 10.3390/molecules27196453 Streszczenie: Drug repurposing in the context of neuroimmunological (NI) investigations is still in its primary stages. Drug repurposing is an important method that bypasses lengthy drug discovery procedures and focuses on discovering new usages for known medications. Neuroimmunological diseases, such as Alzheimer's, Parkinson's, multiple sclerosis, and depression, include various pathologies that result from the interaction between the central nervous system and the immune system. However, the repurposing of NI medications is hindered by the vast amount of information that needs mining. We previously presented Adera1.0, which was capable of text mining PubMed for answering query-based questions. However, Adera1.0 was not able to automatically identify chemical compounds within relevant sentences. To challenge the need for repurposing known medications for neuroimmunological diseases, we built a deep neural network named Adera2.0 to perform drug repurposing. The workflow uses three deep learning networks. The first network is an encoder and its main task is to embed text into matrices. The second network uses a mean squared error (MSE) loss function to predict answers in the form of embedded matrices. The third network, which constitutes the main novelty in our updated workflow, also uses a MSE loss function. Its main usage is to extract compound names from relevant sentences resulting from the previous network. To optimize the network function, we compared eight different designs. We found that a deep neural network consisting of an RNN neural network and a leaky ReLU could achieve 0.0001 loss and 67% sensitivity. Additionally, we validated Adera2.0's ability to predict NI drug usage against the DRUG Repurposing Hub database. These results establish the ability of Adera2.0 to repurpose drug candidates that can shorten the development of the drug cycle. The workflow could be download online.
7/12
Nr opisu: 0000046720 Autorzy: Justyna Paszkiewicz, Anna Leśniak, Mariusz Sacharczuk, Patrycja Teodorowicz. Charakterystyka formalna: projekt badawczy Język publikacji: POL
8/12
Nr opisu: 0000046716 Autorzy: Patrycja Teodorowicz, Mariusz Sacharczuk, Anna Leśniak, Justyna Paszkiewicz. Charakterystyka formalna: projekt badawczy Język publikacji: POL
9/12
Nr opisu: 0000046713 Autorzy: Justyna Paszkiewicz, Mariusz Sacharczuk, Anna Leśniak, Patrycja Teodorowicz. Charakterystyka formalna: projekt badawczy Język publikacji: POL
10/12
Nr opisu: 0000036442 Autorzy: Piotr Poznański, Anna Leśniak, Magdalena Bujalska-Zadrożny, Joanna Strzemecka, Mariusz Sacharczuk. Tytuł pracy: Bidirectional selection for high and low stress-induced analgesia affects G-protein activity Tytuł czasopisma: Szczegóły: 2019, Vol. 144, January 2019, p. 37--42 p-ISSN: 0028-3908 e-ISSN: 1873-7064 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 4.431 Punktacja ministerstwa: 140.000 Praca recenzowana Słowa kluczowe ang.: antinociception ; opioid system ; HA and LA mice ; G-protein activation Uwaga: Kopia dostępna w Sekcji Bibliometrii. DOI: 10.1016/j.neuropharm.2018.10.014 Streszczenie: Mice selected for high (HA) and low (LA) swim stress-induced analgesia (SSIA) are a unique model for studying the genetic background of this phenomenon. HA and LA miceshow substantial differences in the magnitude of the antinociceptive response to stress and when treated with exogenous opioids. However, the direct cause underplaying this distinctive feature has not yet been identified. The current study was designed to investigate the possibility that disturbances in G-protein signaling could explain the divergent response to opioid agonists. Supraspinal and spinal opioid sensitivity was assessed in vivo with intraperitoneal morphine and subsequent thermal stimulus exposure. The level of opioid receptor-mediated G-protein activation was investigated by means of DAMGO and morphine-stimulated [35S]GTPγS assay in the brain and spinal cord homogenates from HA and LA mice. Morphine (3-249 ľmol/kg, i.p) was over 6 - and 3 - times more potent in HA than LA mice in the hot plate and tail-flick assays, respectively. Additionally, HA mice showed elevated ß - endorphin levels in the brain. Enhanced efficacy of agonist-stimulated [35S]GTPγS binding was detected in opioid receptor-rich limbic regions of HA mice like the hypothalamus and hippocampus. Increased G-protein activity also emerged in the thalamus, periaqueductal gray matter and prefrontal cortex. In conclusion, the magnitude of the antinociceptive response to opioids in HA and LA mice is correlated with alterations in G-protein activation in brain regions responsible for integration and descending modulation of nociceptive information as well as at sites governing the emotional response to stressful stimuli.
11/12
Nr opisu: 0000036795 Autorzy: Anna Leśniak, Diana Chmielewska, Piotr Poznański, Magdalena Bujalska-Zadrożny, Joanna Strzemecka, Mariusz Sacharczuk. Tytuł pracy: Divergent Response to Cannabinoid Receptor Stimulation in High and Low Stress-Induced Analgesia Mouse Lines Is Associated with Differential G-Protein Activation Tytuł czasopisma: Szczegóły: 2019, Vol. 404, p. 246--258 p-ISSN: 0306-4522 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 3.056 Punktacja ministerstwa: 140.000 Praca recenzowana Słowa kluczowe ang.: cannabinoid system ; HA and LA mice ; G-protein activation ; WIN55,212-2 Uwaga: Kopia dostępna w Sekcji Bibliometrii. https://www.sciencedirect.com/science/article/abs/pii/S0306452219301174 DOI: 10.1016/j.neuroscience.2019.02.015 Streszczenie: Bidirectional selection of mice for high (HA) and low (LA) swim stress-induced analgesia (SSIA) is associated with a divergent response to opioids. In the current study, we investigated whether the genetic divergence in opioid system activity between HA and LA mice also affects cannabinoid sensitivity. Additionally, we also investigated whether the endocannabinoid system mediates SSIA in these lines. Numerous reports support the existence of pharmacological and molecular interactions between the opioid and cannabinoid systems along the pain pathways, as both systems utilize the same G-protein subtype for signal transduction. Mice from both lines were treated with a non-selective CB1/CB2 agonist, WIN55,212-2 and their behavior was evaluated according to the tetrad paradigm assessing antinociception, catalepsy, hypothermia and locomotor activity. Surprisingly, the engagement of CB1 receptors in SSIA was not confirmed. G-protein activation was studied in different brain regions and the spinal cord in the [35S]GTP?S assay. It was shown that WIN55,212-2 produced more potent antinociception in HA than in LA mice. Also, HA mice displayed stronger cannabinoid-induced catalepsy in the bar test. However, LA mice were more sensitive to the hypothermic effect of WIN55,212-2. The intensity of behavioral responses to WIN55,212-2 was correlated with increased G-protein activation in the periaqueductal gray matter, frontal cortex, striatum and thalamus in HA mice. A weak response to WIN55,212-2 in LA mice could depend on impaired CB2 receptor signaling. In conclusion, differences in both opioid and cannabinoid sensitivity between HA and LA mice could stem from alterations in intracellular second messenger mechanisms involving G-protein activation.
12/12
Nr opisu: 0000035899 Autorzy: Piotr Poznański, Anna Leśniak, Joanna Strzemecka, Mariusz Sacharczuk. Tytuł pracy: Review of research on alcohol dependence in a model of mice selected for high and low stress-induced analgesia Tytuł równoległy: Przegląd badań nad uzależnieniem od alkoholu w modelu myszy selekcjonowanych w kierunku wysokiej i niskiej analgezji postresowej Tytuł czasopisma: Szczegóły: 2018, Vol. 12, issue 3, p. 217--222 p-ISSN: 2353-6942 e-ISSN: 2354-0265 Charakterystyka formalna: artykuł w czasopiśmie polskim Charakterystyka merytoryczna: artykuł przeglądowy Charakterystyka wg MNiSW: artykuł w czasopiśmie bez IF (wykaz MEiN) Język publikacji: ENG Punktacja ministerstwa: 9.000 Praca recenzowana Słowa kluczowe: uzależnienie od alkoholu ; selekcjonowane linie myszy ; układ opioidowy Słowa kluczowe ang.: alcohol abuse ; selected mouse lines ; opioid system https://www.termedia.pl/Review-of-research-on-alcohol-dependence-in-a-model-of-mice-selected-for-high-and-low-stress-induced-analgesia,99,33193,0,1.html DOI: 10.5114/hpc.2018.76747 Streszczenie: Decades of studies on alcohol dependence showed that it is a very complex and multifactorial disorder. Several receptor systems are involved in development and susceptibility to alcohol abuse; however, there are some which play a crucial role in its pathogenesis, e.g. dopaminergic or opioid system. In this paper, an effort is made to explain the role of endogenous opioid system activity in alcohol dependence. To achieve the goal, we use a unique model is used which shows mice lines that are divergently selected for high (HA) and low (LA) stress-induced analgesia. This process allowed for selecting individuals characterised by hyperactive (HA) or hypoactive (LA) opioid system. Basing on the performed experiments, we proved that delta opioid receptors play a critical role in the development of addiction. The most notable achievement is an unspecific reaction of mice with the hyperactive opioid system to naloxone - an unspecific opioid system antagonist, which is currently approved in the pharmacotherapy of dependent patients. Streszczenie: Uzależnienie od alkoholu jest chorobą wieloczynnikową, za której etiologię odpowiedzialnych jest kilka mechanizmów. Wieloletnie badania pozwoliły na zidentyfikowanie kilku układów receptorowych, które są zaangażowane w rozwój oraz podatność do uzależnienia od alkoholu, jednakże niektóre z nich odgrywają krytyczną rolę w jego patogenezie np. układ dopaminergiczny lub opioidowy. W niniejszym artykule przedstawiamy wyniki badań, których celem było określenie roli endogennej aktywności układu opioidowego w uzależnieniu od alkoholu. Do realizacji tego celu wykorzystano unikalny model myszy selekcjonowanych w kierunku wysokiej (HA) oraz niskiej (LA) analgezji postresowej. Proces selekcji pozwolił na wybranie osobników charakteryzujących się wysoką (HA) oraz niską (LA) aktywnością układu opioidowego. Na podstawie przeprowadzonych eksperymentów, udowodniliśmy krytyczną rolę receptorów opioidowych typu delta w rozwoju uzależnienia od alkoholu. Godnym uwagi osiągnięciem było wykazanie niespecyficznej reakcji myszy o wysokiej aktywności układu opioidowego na nalokson - niespecyficznego antagonistę receptorów opioidowych, który jest aktualnie używany w farmakoterapii uzależnionych pacjentów.
