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Autorzy: , . Tytuł pracy: p-ISSN: original-article p-ISSN: 995199 p-ISSN: 009995.300 p-ISSN: 2076-0817 p-ISSN: 003 p-ISSN: Mycobacterium bovis Transmission between Cattle and a Farmer in Central Poland p-ISSN: Pathogens p-ISSN: 2022 p-ISSN: 2076-0817 p-ISSN: 2022/2023 p-ISSN: 10.3390/pathogens11101170 p-ISSN: Weiner, Marcin p-ISSN: cattle p-ISSN: FINAL_PUBLISHED p-ISSN: Zoonoses have recently become an increasing public health problem. Zoonoses are estimated to account for 60% of all emerging infectious diseases. One particularly important zoonosis is human tuberculosis, especially tuberculosis due to Mycobacterium bovis (M. bovis), which is naturally resistant to pyrazinamide (PZA). Material and Methods: The patient had a pulmonary form of tuberculosis accompanied by a cough and fever. At the same time, the disease was also confirmed in 20 out of 25 cattle on the farm. The clinical specimen (sputum) was examined in accordance with the European Union (EU) laboratories' methodology. Tissue materials from cattle were verified in the National Veterinary Research Institute (NVRI), in the Bovine tuberculosis (BTB) Reference Laboratory, Pulawy, Poland and tested in accordance with the guidelines for the laboratory diagnosis of BTB. Results: All M. bovis isolates represented one spoligotype, SB0120. The results of mycobacterial interspersed repetitive unit variable number tandem repeat (MIRU-VNTR) evaluation showed the same genetic pattern. Conclusion: Findings from this study suggest the first confirmed interspecific transmission of Mycobacterium bovis, between a farmer and his cattle, in Poland. Present findings support the increasing concern regarding zoonotic TB that has been highlighted elsewhere. p-ISSN: human p-ISSN: Mycobacterium bovis p-ISSN: SB0120 p-ISSN: transmission p-ISSN: Poland e-ISSN: Oryginalny artykuł naukowyACZartykuł w czasopiśmie zagranicznym3.700IF e-ISSN: 998999100.0000100.000PUNKTACJA KBNPUNKTACJA MINISTERSTWALISTA FILADELFIJSKAIMPACT FACTOR100.000PUNKTACJA UWM e-ISSN: 009899.000 e-ISSN: Q e-ISSN: 003 e-ISSN: Vol. 11 e-ISSN: CC-BY Charakterystyka formalna: Słowa kluczowe ang.: Streszczenie:
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Autorzy: , , . Szczegóły: Charakterystyka wg MNiSW: Słowa kluczowe ang.: Inne bazy podające opis:
ent ante-mortem testing protocols (the tuberculin skin and Enferplex Camelid TB tests) to identify TB-free alpaca herds and individuals for export. Our research and the available literature indicate that the alpaca (Vicugna pacos) is extremely susceptible to Mycobacterium bovis infection, and that testing periodicity fails to take into account that animals do not manifest disease symptoms for a long time. The skin test failed to identify Mycobacterium bovis infection in two alpacas prior to their movement from the UK to Poland. The animals were purchased by a breeding centre in Poland, and were then shown at an international animal exhibition. The last owner of the alpacas before their deaths from TB bought the infected animals unwittingly in order to run rehabilitation activities with disabled children on his farm. Material and Methods: Thoracic lymph node, lung and liver tissue samples obtained at necropsy were examined histopathologically after Ziehl-Neelsen staining. Tissue samples were homogenised and mycobacteria present there were cultured on Stonebrink's medium during a 6-week incubation. A commercial test using polymorphism of the chromosomal direct repeat region provided species identification and additional identification was by spacer oligonucleotide typing and mycobacteria interspersed repetitive unit-variable number tandem repeat analysis with a gel electrophoresis protocol. Results: The microbiological examination confirmed multiorgan TB caused by the SB0666 spoligotype of Mycobacterium bovis. Conclusion: Due to the suboptimal performance of current diagnostic tests for TB in alpacas, there is a risk that infected animals may be moved unwittingly. A risk of TB spread associated with the international movement of alpacas is implied by this study.^aEnferplex Camelid TB test^aMycobacterium bovis^atuberculin skin test
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Nr opisu: iśmie polskim1.744IF^a997156^b998599140.0000140.000PUNKTACJA KBNPUNKTACJA MINISTERSTWALISTA FILADELFIJSKAIMPACT FACTOR40.000PUNKTACJA UWM^a009997.256^b009859.000^c009999.000^d009959.000202020202020Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Po00000393610000001042AOartykuł oryginalny naukowyPUBLIKACJAPEŁNA PUBLIKACJAAAartykuł w czasopiśmie z IF (wykaz MNiSW)AFILIACJA PODANAENGhttps://content.sciendo.com/view/journals/jvetres/64/1/article-p45.xmlPRACA RECENZOWANA100^a2450-7393^bQ^e2450-7393^fA^hABC^iX^jXY^kQ013155^a003^b003^c2020-04-20, 00:04^d2021-07-02, 12:00^e3426850235^f3324029039^aMolecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland^aJournal of Veterinary Research^a2020^bVol. 64^cissue 1^dp. 45--50^a2450-7393^b2450-8608^a2019/2020^a10.2478/jvetres-2020-0003^aWeiner, Marcin^cx^acattle^aKopia dostępna w Sekcji Bibliometrii.^aFINAL_PUBLISHED^bCC-BY-NC-ND^cAT_PUBLICATION^eOPEN_JOURNAL^aIntroduction. Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008-2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks. Material and Methods. The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province. Results. Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.^abovine tuberculosis^aMycobacterium bovis^aPolandHED^bCC-BY-NC-ND^cAT_PUBLICATION^eOPEN_JOURNAL^aIntroduction. Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008-2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks. Material and Methods. The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province. Results. Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.^abovine tuberculosis^aMycobacterium bovis^aPoland Autorzy: , , , 009859.000 Q 003 Vol. 64 2450-8608 CC-BY-NC-ND 009997.2562450-7393003Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in PolandJournal of Veterinary Research20202450-73932019/202010.2478/jvetres-2020-0003Weiner, MarcincattleKopia dostępna w Sekcji Bibliometrii.FINAL_PUBLISHEDIntroduction. Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008-2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks. Material and Methods. The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province. Results. Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.bovine tuberculosisMycobacterium bovisPoland. Tytuł pracy: Szczegóły: 009997.2562450-7393003Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in PolandJournal of Veterinary Research20202450-73932019/202010.2478/jvetres-2020-0003Weiner, MarcincattleKopia dostępna w Sekcji Bibliometrii.FINAL_PUBLISHEDIntroduction. Since 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008-2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the study was the molecular characterisation of the pertinent M. bovis/caprae strains and determination of the epidemiological relationship of various bTB outbreaks. Material and Methods. The material for microbiological tests came from 119 cattle (Bos taurus) from nine herds located in five provinces, neighbouring the Masovian Province. Results. Laboratory tests of tissue material gave results confirming tuberculosis in 54 (45%) animals. All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds.bovine tuberculosisMycobacterium bovisPoland, 009859.000, Q, 003, Vol. 64, 2450-8608, CC-BY-NC-ND, 009999.000, 2020-04-20, 00:04, issue 1, x, AT_PUBLICATION, 009959.000202020202020Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Po00000393610000001042AOartykuł oryginalny naukowyPUBLIKACJAPEŁNA PUBLIKACJAAAartykuł w czasopiśmie z IF (wykaz MNiSW)AFILIACJA PODANAENGhttps://content.sciendo.com/view/journals/jvetres/64/1/article-p45.xmlPRACA RECENZOWANA100, 2021-07-02, 12:00, p. 45--502450-73933426850235OPEN_JOURNAL Charakterystyka merytoryczna: Język publikacji: Wskaźnik Impact Factor ISI: Punktacja ministerstwa: Praca recenzowana Słowa kluczowe ang.: