Bibliografia publikacji pracowników
Państwowej Szkoły Wyższej w Białej Podlaskiej
Baza tworzona przez Bibliotekę Akademii Bialskiej im. Jana Pawła II.
Zapytanie:
ATOMIC FORCE MICROSCOPY Liczba odnalezionych rekordów: 4
Przejście do opcji zmiany formatu | Wyświetl/ukryj etykiety | Wyświetlenie wyników w wersji do druku | Pobranie pliku do edytora | Nowe wyszukiwanie Streszczenie: Small molecules containing quinone and/or oxime moieties have been found as promising anti-fungal agents. One of them is 4-AN, a recently reported potent anti-Candida compound, which inhibits the formation of hyphae, decreases the level of cellular phosphoproteome, and finally shows no toxicity towards human erythrocytes and zebrafish embryos. Here, further research on 4-AN is presented. The results revealed that the compound: (i) Kills Candida clinical isolates, including these with developed antibiotic resistance, (ii) affects mature biofilm, and (iii) moderately disrupts membrane permeability. Atomic force microscopy studies revealed a slight influence of 4-AN on the cell surface architecture. 4-AN was also shown to inhibit multiple various protein kinases, a characteristic shared by most of the ATP-competitive inhibitors. The presented compound can be used in novel strategies in the fight against candidiasis, and reversible protein phosphorylation should be taken into consideration as a target in designing these strategies.
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Nr opisu: 0000035343 Autorzy: Monika Janeczko, Konrad Kubiński, Aleksandra Martyna, Angelika Muzyczka, Anna Boguszewska-Czubara, Sławomir Czernik, Małgorzata Tokarska-Rodak, Marta Chwedczuk, Oleg M. Demchuk, Hieronim Golczyk, Maciej Masłyk. Tytuł pracy: 1,4-Naphthoquinone derivatives potently suppress Candida albicans growth, inhibit formation of hyphae and show no toxicity toward zebrafish embryos Tytuł czasopisma: Szczegóły: 2018, Vol. 67, issue 4, p. 598--609 p-ISSN: 0022-2615 Charakterystyka formalna: artykuł w czasopiśmie zagranicznym Charakterystyka merytoryczna: artykuł oryginalny naukowy Charakterystyka wg MNiSW: artykuł w czasopiśmie z IF (wykaz MEiN) Język publikacji: ENG Wskaźnik Impact Factor ISI: 1.926 Punktacja ministerstwa: 25.000 Praca recenzowana Słowa kluczowe ang.: Naphthoquinones ; Candida albicans ; hyphae ; biofilm ; atomic force microscopy ; zebrafish Uwaga: Kopia dostępna w Sekcji Bibliometrii. http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000700 DOI: 10.1099/jmm.0.000700 Streszczenie: Purpose. In this study, we applied various assays to find new activities of 1,4-naphthoquinone derivatives for potential anti-Candida albicans applications. Methodology. These assays determined (a) the antimicrobial effect on growth/cell multiplication in fungal cultures, (b) the effect on formation of hyphae and biofilm, (c) the influence on cell membrane integrity, (d) the effect on cell morphology using atomic force microscopy, and (e) toxicity against zebrafish embryos. We have demonstrated the activity of these compounds against different Candida species and clinical isolates of C. albicans. Key findings. 1,4-Naphthoquinones significantly affected fungal strains at 8-250mg-1 of MIC. Interestingly, at concentrations below MICs, the chemicals showed effectiveness in inhibition of hyphal formation and cell aggregation in Candida. Of note, atomic force microscopy (AFM) analysis revealed an influence of the compounds on cell morphological properties. However, at low concentrations (0.8-31.2 mg-1), it did not exert any evident toxic effects on zebrafish embryos. Conclusions. Our research has evidenced the effectiveness of 1,4-naphthoquinones as potential anti-Candida agents.
